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Inventi Impact: Biomedical Analysis

Articles

  • Inventi:pba/21643/17
    Performance Evaluation of the Touchscreen-Based Museâ?¢ Auto CD4/CD4% Single-Platform System for CD4 T Cell Numeration in Absolute Number and in Percentage Using Blood Samples from Children and Adult Patients Living in the Central African Republic
    01-Jan-1970 Research 2017 : January - March
    Christian Diamant Mossoro-Kpinde, Andre Kouabosso, Ralph-Sydney Mboumba Bouassa, Jean De Dieu Longo, Edouard Kokanzo, Rosine Feissona, Gerard Gresenguet, Laurent Belec

    Background: The new microcapillary and fluorescence-based EC IVD-qualified Museââ??¢ Auto CD4/CD4% singleplatform\nassay (EMD Millipore Corporation, Merck Life Sciences, KGaA, Darmstadt, Germany) for CD4 T cell numeration\nin absolute number and in percentage was evaluated using Central African patientsââ?¬â?¢ samples compared against the\nreference EC IVD-qualified BD FACSCount (Bectonââ?¬â??Dickinson, USA) flow cytometer.\nMethods: EDTA-blood samples from 124 adults, 10 adolescents, 13 children and 3 infants were tested in parallel at 2\nreference laboratories in Bangui.\nResults: The Museââ??¢ technique was highly reproducible, with low intra- and inter-run variabilities less than 15%. CD4\nT cell counts of Museââ??¢ and BD FACSCount in absolute number and percentage were highly correlated (r2 = 0.99 and\n0.98, respectively). The mean absolute bias between Museââ??¢ and BD FACSCount cells in absolute number and percentage\nwere âË?â??5.91 cells/Ã?¼l (95% CI âË?â??20.90 to 9.08) with limits of agreement from âË?â??77.50 to 202.40 cells/Ã?¼l, and +1.69\n

    How to Cite this Article
    CC Compliant Citation: Mossoro-Kpinde, Christian Diamant, et al. \"Performance evaluation of the touchscreen-based Museâ?¢ Auto CD4/\nCD4% single-platform system for CD4 T cell numeration in absolute number and in percentage using blood samples from children and\nadult patients living in the Central African Republic.\" Journal of Translational Medicine 14.1 (2016): 326, DOI 10.1186/\ns12967-016-1082-7, https://creativecommons.org/licenses/by/4.0/.
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